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Innate immunity against bacterial pathogens

Friday 29 January 2016

Innate immunity against bacterial pathogens
Prof.dr Jos van Putten

Project
Microbial pathogens impose a major health problem as illustrated by the
(re-)emergence of infectious diseases and their increasingly recognized role in auto-immunity and tumour development. The research of our group is focused on the unraveling of the molecular mechanisms that play a role in the pathogenesis of bacterial infections and the associated pathobiology. This project focus on the evolution and function of the innate host response towards bacteria with emphasis on Toll-like receptors. These receptors play a key role in regulation of the defense against bacteria and regulate B- and T-cell responses. The work follows a comparative biology approach and involves the use of a combination of molecular biology, biochemical, cell biological and immunological techniques and is carried out in an international setting. Results provide insight in the function and diversity of the innate immune system among species and may lead to the development of novel vaccine adjuvants and immunomodulatory agents.

Techniques
 Genetic engineering of eukaryotic cells including DNA/RNA isolation, electrophoresis, PCR, cloning, mutagenesis, sequence-analysis, real-time PCR, transfection and chimeric receptor design;
 Molecular microbiology techniques including culture of (pathogenic) bacteria under ML-II conditions, construction of mutants, expression and purification of (recombinant) TLR ligands (LPS, flagellin), (affinity) chromatography;
 Immunological techniques such as isolation of cells, Western blotting, ELISA, FACS and cytokine gene expression analysis;
 Cell biology methods such as cell culture of primary cells and cell lines, infection assays, immunohistochemistry, labeling and advanced (confocal) microscopy.

Duration
9 months (6 months)

Contact
Prof.dr. Jos van Putten, Tel: 030 - 2534344, j.vanputten@uu.nl