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Bacterial adaption mechanism

Friday 29 January 2016

Bacterial adaptation mechanism

Project
Metabolic activity is essential in all living organisms to generate energy for cellular biosynthesis. Unlike plant and animal cells, most bacteria are exposed to a constantly changing physical and chemical environment. In order to survive or to cause disease bacterial communities must be able to adapt their metabolism in response to changes in the environmental conditions. This project aims to discover environmental signals and molecular adaptation mechanisms that enable the survival and virulence of intestinal bacteria. The work involves the use of a combination of molecular biology, biochemistry, microbiological and tissue culture techniques and is carried out in an international setting. Results increase understanding of host pathogen interactions and are important to develop novel therapeutic, prevention and diagnostic strategies.

Techniques
Genetic engineering of bacteria, PCR, sequence-analysis;
Microbiological techniques, generation of automatic growth curves under different gas atmospheric conditions, motility and chemotaxis assays;
Gene expression studies, transcriptomics and proteomics analysis, real time RT-PCR, enzyme assays, promoter fluorescent reporter assays;
Protein-protein or DNA/RNA interactions, protein purification, SDS-PAGE Western and Far-Western blotting, electro mobility shift assays;
Metabolomics and fatty acids analysis, HPLC-MS Analysis;
Tissue culture invasion and adaptation assays


Duration
9 months (6 months)

Contact
Dr. Marc Wösten, Tel: 030 - 2534791, m.wosten@uu.nl