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Neutrophil subsets in animal models of cancer

Tuesday 7 July 2015

Neutrophil subsets in animal models of cancer
Recent work in our lab showed that in extraordinary conditions (e.g. trauma, LPS challenge, exercise challenge), distinct subsets of human neutrophilic granulocytes appear in the blood. These subsets have altered phenotypes and different functionalities, which we are just starting to unravel. For example, one subset can suppress T cell proliferation. To aid our understanding, we also want to follow these subsets in animal models. So far we have been working with acute models such as LPS challenged mice. In this project we want to investigate whether chronic inflammation can also induce subsets with similar functionality in mice. Cancer is a typical example of low-grade persistent inflammation and therefore an ideal model. Especially since Myeloid-derived suppressor cells (MDSC), which encompass granulocytes with immunosuppressive functions, have become a hot topic in the field of cancer research. In the Villin-CreERT2; APC-loxPHET colorectal cancer model, you will isolate neutrophils and perform ex vivo assays to determine the inflammatory profile and performance of key functions (e.g. migration, killing, T cell interactions). Subsequent experiments will hopefully tell us more about the function of the immune system in cancer progression.

Techniques
FACS, (confocal) microscopy, isolation of lymphocytes and phagocytes from peripheral blood, cell culture, functional assays ex vivo, in vivo animal experiments, two-photon intravital microscopy

Duration
6 or 9 months

Contact
Dr. Nienke Vrisekoop N.Vrisekoop@umcutrecht.nl