Thi Kim Anh Nguyen
Monday 3 October 2011
Towards the use of CD1 presented mycobacterial lipids in vaccines
Promotor: Prof. dr W. van Eden
Defence: 3 October 2011
The work presented in this thesis addresses the potential of mycobacterial lipid vaccination against mycobacterial infections. Cattle, natural hosts of M. avium subsp paratuberculosis (MAP) and M. bovis were used throughout this thesis because they are an important target species, as well as an animal model for human tuberculosis. Since CD1 is likely to play a role in presentation of mycobacterial lipid antigens, the presence and expression of bovine CD1 genes and their function in natural infection was investigated. CD1 restricted lipid antigen specific T cell responsiveness was studied in naturally infected cattle and led to the identification of the glycolipid glucose monomycolate (GMM) as an immunodominant lipid compound of MAP. This immunogenic mycobacterial lipid was subsequently used in two different vaccine formulations to examine induction of specific immune responses.