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Characterization of isoforms of SIRL-1

Thursday 3 February 2011

Prof.dr Linde Meyaard

Project
The human Signal Inhibitory Receptor on Leukocytes (SIRL)-1 belongs to the growing family of inhibitory immune receptors, implied in the regulation of phagocytes. Antibody cross-linking of SIRL-1 in vitro is capable of inhibiting cellular phagocyte functions. Therefore, SIRL-1 is likely to have key roles in the function of these cells, particularly in a microenvironment where these cells are recruited and activated.

We have made a panel of monoclonal antibodies recognzing SIRL-1 . Some of these recognize a structure on activated lymphocytes, while others do not. The goal of the current project is to characterize the differences between phagocyte and lymphocyte-expressed SIRL-1 and to reveal the functional consequences of this differential expression. Furthermore, we will explore if SIRL-1 can be shed from the membrane of SIRL-1 expressing cells and can be detected in supernatants of cultured cells as well as biological samples such as plasma and urine from healthy controls and patients

Techniques
Western blot, FACS, ELISA, cellsorting , RNA isolatie,RT-PCR, cloning, sequencing, cell-culture, isolation of lymphocytes and phagocytes from peripheral blood

Duration
6 or 9 months

Contact
Linde Meyaard, l.meyaard@umcutrecht.nl
Dr. Kristin Denzer, k.denzer@umcutrecht.nl, 088 75 643 68

References
T.A.M. Steevels, R.J. Lebbink, G.H.A.Westerlaken, P.J. Coffer and L. Meyaard. 2010. Signal Inhibitory Receptor on Leukocytes-1 (SIRL-1) is a novel functional inhibitory immune receptor expressed on human phagocytes. J. Immunol. 184:4741-4748.


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