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Molecular biology, pathogenesis and evolution of RNA viruses

Tuesday 5 May 2009

Molecular biology, pathogenesis and evolution of RNA viruses

Prof. dr F.J.M. van Kuppeveld

Project
The general aim of our research is the understanding of the infection process of viruses at the level of the cell, the organism and the population, and the development of strategies for the diagnosis of and protection against these infections. With respect to the viruses, we focus on RNA viruses with particular attention to picornaviruses, coronaviruses, and influenza A viruses. These viruses cause important diseases in animals and humans. Central topics in our research program are: virus structure and assembly; replication; cell entry; the functions of structural and non-structural proteins in the infection process and in viral pathogenesis; the interactions of the virus with the host and the host immune system; development of vaccines and antivirals.

Techniques
Virtually all classical and modern molecular-virological, cell biological techniques and immunological techniques are employed:

Tissue culture. Propagation, titration and purification of viruses
State-of-the-art recombinant DNA and Protein analysis
Structural analysis of proteins
Bioinformatics
Heterologous expression of genes in bacterial and eukaryotic cells
Reverse genetics (genetic modification) of RNA and DNA viruses
Microscopy (confocal immunofluorescence microscopy; (cryo)electron microscopy)
Life cell and whole animal imaging
Functional genomics (RNAi , haploid genetic screening, Crispr/Cas9 genome editing)
Glycovirology (glycobiological techniques; glycan arrays, Octet Biolayer interferometry)

Duration
6 or 9 months

Contact (application for internships):
Mevr. Anja Streefkerk-Veldt; 030 2532485; E a.j.streefkerk-veldt@uu.nl / virology@vet.uu.nl

Contact (scientific and practical information)
Dr R.J. de Groot; 030 - 2531463, R.J.deGroot@uu.nl