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Vaccine development

Tuesday 5 May 2009

Prof.dr Jos van Putten

Infection prevention is the best solution to combat the (re-)emergence of infectious diseases and antibiotic resistance. Our group aims to identify candidate vaccine components for the major bacterial intestinal and other mucosal pathogens. This project involves the identification, cloning and expression of vaccine antigens, and evaluation of their immunogenicity as potential vaccine constituent. The work involves the use of a combination of molecular biology, biochemistry and immunological techniques and is carried out in an international setting and partially in collaboration with industry. The results of the experiments may lead to the development of novel vaccines.

Genetic engineering of bacteria including DNA/RNA isolation, restriction-enzyme analysis, electrophoresis, Southern blotting, PCR, cloning, mutagenesis, sequence-analysis, Northern blotting, DNA footprinting, real-time PCR, RT-PCR and transfection;

Biochemical methods including protein and carbohydrate purification, SDS-PAGE, (affinity) chromatography;

Immunological techniques such as isolation of cells, Western blotting, ELISA and FACS analysis.

9 months (6 months)

Prof.dr Jos van Putten, Tel: 030 - 2534344, j.vanputten@uu.nl