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Innate immunity against bacterial pathogens

Tuesday 5 May 2009

Prof.dr Jos van Putten

Microbial pathogens impose a major health problem as illustrated by the
(re-)emergence of infectious diseases and their increasingly recognized role in auto-immunity and tumour development. The research of our group is focused on the unraveling of the molecular mechanisms that play a role in the pathogenesis of bacterial infections and the associated pathobiology. In this project the innate host response towards infection is investigated, with emphasis on the role of recognition of bacterial components by innate receptors such as Toll-like receptors. These receptors play a key role in regulation of the defense against bacteria and regulate B- and T-cell responses. The work involves the use of a combination of molecular biology, biochemical, cell biological and immunological techniques and is carried out in an international setting. The results of the experiments may lead to the development of novel vaccine adjuvants and immunomodulatory agents.

Genetic engineering of eukaryotic cells including DNA/RNA isolation, restriction-enzyme analysis, electrophoresis, PCR, cloning, mutagenesis, sequence-analysis, real-time PCR, RT-PCR and transfection;

Biochemical methods including protein purification, SDS-PAGE, (affinity) chromatography;

Immunological techniques such as isolation of cells, Western blotting, ELISA and FACS analysis;

Cell biology methods such as cell culture, infection assays, immunohistochemistry, labeling and advanced (confocal) microscopy.

9 months (6 months)

Prof.dr. Jos van Putten, Tel: 030 - 2534344, j.vanputten@uu.nl