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Bacterial virulence strategies

Tuesday 5 May 2009

Prof.dr Jos van Putten

Microbial pathogens impose a major health problem as illustrated by the
(re-)emergence of infectious diseases and their increasingly recognized role in auto-immunity and tumour development. The research of our group is focused on the unraveling of the molecular mechanisms that play a role in the pathogenesis of bacterial infections and the associated pathobiology. This project aims to decipher how the most important bacterial intestinal pathogens adhere to, invade and survive in human intestinal epothelial cells and professional phagocytes. The project involves the identification of novel bacterial virulence factors and host receptors and unraveling of bacterial invasion mechanisms. The work involves the use of a combination of molecular biology, cell biology and immunology techniques and is carried out in an international setting. The results of the experiments may lead to the development of novel infection intervention and prevention methods such as vaccine development and better treatment of infection associated pathobiology.

Genetic engineering of bacteria and eukaryotic cells including DNA/RNA isolation, restriction-enzyme analysis, electrophoresis, Southern blotting, PCR, cloning, mutagenesis, sequence-analysis, Northern blotting, real-time PCR, RT-PCR and transfection;

Biochemical methods such as 2D-electrophoresis and proteomics;

Immunological techniques such as isolation of cells, Western blotting, ELISA and FACS analysis;

Cell biology methods such as cell culture, infection assays, immunohistochemistry and advanced (confocal)microscopy.

9 months (6 months)

Prof.dr. Jos van Putten, Tel: 030 - 2534344, j.vanputten@uu.nl