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Bacterial gene regulation

Tuesday 5 May 2009

Prof.dr Jos van Putten

Microbial pathogens impose a major health problem as illustrated by the
(re-)emergence of infectious diseases and their increasingly recognized role in auto-immunity and tumour development. The research of our group is focused on the unraveling of the molecular mechanisms that play a role in the pathogenesis of bacterial infections and the associated pathobiology. This project aims to discover how bacterial intestinal pathogens can adopt to the different growth environments and availability of nutrients. The work involves the use of a combination of molecular biology, microbiological and immunological techniques and is carried out in an international setting. The results of the experiments may lead to the development of novel infection intervention and prevention methods such as vaccine development and better treatment of infection associated pathobiology.

Genetic engineering of bacteria including DNA/RNA isolation, restriction-enzyme analysis, electrophoresis, Southern blotting, PCR, cloning, mutagenesis, sequence-analysis, Northern blotting, DNA footprinting, real-time PCR, RT-PCR;

Biochemical methods including (recombinant) protein expression and purification, SDS-PAGE, (affinity) chromatography;

Immunological techniques such as Western blotting.

9 months (6 months)

Prof.dr Jos van Putten, Tel: 030 - 2534344, j.vanputten@uu.nl